Top: cell-type shares inferred from 35 PBMC bulk RNA-seq samples, against the shares measured by
single-cell RNA-seq of the same samples, per lineage. Filled points and solid lines: Ladon; hollow points and
dashed lines: the chosen comparator. Lines: least-squares fits; r: Pearson correlation. Horizontal axes differ by
lineage and do not start at 0; vertical axes start at 0 and stay fixed when the comparator changes. Ladon's estimates track the measured shares in every
lineage. Bottom: accuracy of each method on the same samples, averaged over the five lineages: Pearson r between
inferred and measured shares, and the share of sample pairs put in the correct order (0.5 is chance). Points:
estimates; bars: 95% confidence intervals from resampling donors. Methods are grouped by their reference: published signatures from bulk
RNA-seq of sorted cells, or single-cell data of the other PBMC studies (the same samples for all methods in the group).
Ladon is the most accurate by both measures. Choose a comparator above or click a method in the
accuracy chart; hover a point to follow that sample across lineages and see its whole composition.